IN VITRO PROPAGATION OF PLATELETS FROM EMBRYONIC STEM CELLS WITH C. PAPAYA LEAF EXTRACT

Objective: The depletion of platelets in patients under different clinical conditions like thrombocytopenia is often associated with a significant mortality factor. Alternative modes platelet propagation from other resources, including stem cells, are evaluated vitro conditions. Embryonic (ES) cells can proliferate and differentiate into various cell types. This study the generation functional embryonic C. papaya leaf extract through controlled sequential pathway to meet demand.
 Methods: In culture was carried out help growth factors suitable medium. differentiated megakaryocytes were analyzed by staining, flow cytometer studies. normal compared Stem cells.
 Results: derived morphologically identical isolated peripheral blood, number viable cytometer. distinctive two waves production represented primitive definitive hematopoiesis.
 Conclusion: preliminary milestone extract. differentiation ESCs megakaryocyte-mediated has shown valuable information on positive effects that be used for large-scale production.